Comparison of the Effectiveness of Anredera cordifolia Ethanol Extract and Fusidic Acid on Skin Incision Wound Healing in Male Rattus norvegicus Sprague-Dawley Strain
DOI:
https://doi.org/10.53366/jimki.v12i2.1008Keywords:
binahong leaf extract, Anredera cordifolia, fusidic acid, incision wound healing, Rattus norvegicus, Sprague-DawleyAbstract
Background: Incision wound healing is a complex biological process involving hemostasis, inflammation, proliferation, and remodeling phases. Fusidic acid is widely used as a topical therapy due to its antibacterial activity; however, prolonged use may lead to bacterial resistance. Anredera cordifolia (binahong) contains flavonoids, saponins, and tannins, which have been reported to exhibit anti-inflammatory, antioxidant, and collagen-stimulating effects, making it a potential alternative therapy.
Method: This study employed a true experimental design with a post-test only control group involving 25 male Rattus norvegicus of the Sprague-Dawley strain, divided into four groups: P0 (fusidic acid), and P1, P2, P3 treated with topical ethanol extract of binahong leaves at concentrations of 10%, 20%, and 40%, respectively. Wound healing was evaluated on days 3, 7, and 14 by measuring wound contraction. Data were analyzed using One-Way ANOVA followed by LSD post hoc test.
Results: No significant difference (p > 0.05) was found between P1, P2, and P3 compared to the positive control (P0).
Discussion: The highest effectiveness observed at 40% concentration is presumed to be associated with the anti-inflammatory activity and fibroblast proliferation stimulation from its secondary metabolites, which accelerate granulation tissue formation. These findings indicate that binahong extract has comparable wound healing potential to fusidic acid as a topical agent.
Conclusion: The ethanol extract of Anredera cordifolia leaves shows wound healing effectiveness comparable to fusidic acid and, at higher concentrations, may even surpass it in promoting skin incision healing in white rats. Binahong can be considered a promising herbal-based alternative for topical wound management.
References
1. Zulkefli, N., Che Zahari, C.N.M., Sayuti, N.H., Kamarudin, A.A., Saad, N., Hamezah, H.S., Bunawan, H., Baharum, S.N., Mediani, A., Ahmed, Q.U., Ismail, A.F.H. & Sarian, M.N. Flavonoids as potential wound-healing molecules: Emphasis on pathways perspective. International Journal of Molecular Sciences. 2023; 24(5): 4607.
2. Cañedo-Dorantes, L. & Cañedo-Ayala, M. Skin acute wound healing: A comprehensive review. International Journal of Inflammation. 2019; 2019(1): 3706315.
3. Almadani, Y.H., Vorstenbosch, J., Davison, P.G. & Murphy, A.M. Wound healing: A comprehensive review. Seminars in Plastic Surgery. 2021; 35(3): 141–144. Thieme Medical Publishers, Inc.
4. Halim, H.A., Ratnah, S. & Abdullah, T.
Skrining fitokimia dan potensi antibakteri ekstrak daun Binahong (Anredera cordifolia Ten. Steenis) terhadap Staphylococcus aureus dan Escherichia coli. Jurnal Labora Medika. 2022; 6(2): 49–52.
5. Basyuni, M., Yulianti Anasta Br Ginting, P. & Lesmana, I. Phytochemical analysis of Binahong (Anredera cordifolia) leaves extract to inhibit in vitro growth of Aeromonas hydrophila. AIP Conference Proceedings. 2017; 1904(1): 020072
6. Yudhantara SM, Rohmawati L. Pengaruh konsentrasi pelarut terhadap kandungan flavonoid total daun binahong (Anredera cordifolia) menggunakan metode microwave assisted extraction. J Biotropical Res Nat Technol. 2023;1(2):92–97.
7. Hasbullah UHA. Kandungan senyawa saponin pada daun, batang, dan umbi tanaman binahong (Anredera cordifolia (Ten.) Steenis). Planta Tropika. 2016;4(1):20–24.
8. Golebiewska EM, Poole AW. Platelet secretion: From haemostasis to wound healing and beyond. Blood Rev. 2015;29(3):153–162.
9. Zaidi A, Green L. Physiology of haemostasis. Anaesthesia & Intensive Care Medicine. 2019;20(3):152–158.
10. Sherwood L. Human Physiology: From Cells to Systems. 9th ed. Boston: Cengage Learning; 2018. p. 152–4.
11. Kementerian Kesehatan Republik Indonesia. Laporan Nasional Riskesdas 2018. Jakarta: Lembaga Penerbit Badan Penelitian dan Pengembangan Kesehatan; 2019.
12. Kementerian Kesehatan Republik Indonesia. Laporan Riset Kesehatan Dasar (Riskesdas) 2013. Jakarta: Badan Penelitian dan Pengembangan Kesehatan; 2013.
13. Susanti G. Efek anti inflamasi ekstrak daun binahong (Anredera cordifolia (Ten.) Steenis) topikal terhadap jumlah PMN neutrofil pada tikus jantan Sprague Dawley. Jurnal Kesehatan. 2017;8(3):351–7.
14. Syawalien WA, Santoso APR. Aktivitas antibakteri ekstrak etanol daun binahong (Anredera cordifolia) terhadap Staphylococcus aureus dan Pseudomonas aeruginosa. Detector: Jurnal Inovasi Riset Ilmu Kesehatan.
15. Susanti H. Total phenolic content and antioxidant activities of binahong (Anredera cordifolia). J Kedokt Kesehat Indones. 2024;10(2).
16. Guyton AC, Hall JE. Textbook of Medical Physiology. 14th ed. Philadelphia: Elsevier; 2021. p. 1027–1030.
17. Robbins SL, Cotran RS, Kumar V. Robbins and Cotran Pathologic Basis of Disease. 10th ed. Philadelphia: Elsevier; 2021. p. 91–95.
Additional Files
Published
Issue
Section
License
Copyright (c) 2025 Alvina Christy Maretta, Waluyo Rudiyanto, Anisa Nuraisa Jausal, Hendra Tarigan Sibero, Sugeng Dwi Hastono
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.